# Glossary ## A ### alignment ## B ## C ### csv [Comma Separated Values (CSV)](https://en.wikipedia.org/wiki/Comma-separated_values) is an ASCII plain-text file format where columns of data are separated by commas. ## D ### DNA [Deoxyribonucleic acid (DNA)](https://en.wikipedia.org/wiki/DNA) is a double-helix polymer of four molecules Cytosine, Guanine, Adenine and Thymine. It is the genetic material of the vast majority of organisms (some viruses use [Ribonucleic Acid (RNA)](https://en.wikipedia.org/wiki/RNA) as their genetic material). ## E ## F ### flattening The process of removing [tilt](glossary.md#tilt) from an image. ## G ### Git [Git](https://git-scm.com) is a free, open source version control system for managing software projects and development. ### GitHub [GitHub](https://www.github.com) is a website for sharing and collaboratively working on software that is version controlled using [Git](glossary.md#git). ### grains The name given to DNA/RNA/protein structures observed in scans. ## H ## I ## J ## K ## L ## M ## N ## O ## P ### Python [Python](https://www.python.org/) is the high-level interpreted programming language in which TopoStats is written. ## Q ## R ## S ### scars Artefacts seen in some scans that can be removed during processing. Typically they appear as horizontal high bands. These are removed and the missing values interpolated. ### skeleton The single-pixel width outline of a molecule after a [grain](glossary.md#grain) has undergone [tracing](glossary.md#tracing). ## T ### tilt ### tracing The processing step where by a [grain](glossary.md#grain) is reduced to a single pixel or skeleton. Typically a number of statistics on the shape, length and curvature are calculated after molecules have been traced. ## U ## V ## W ## X ## Y ## Z