Source code for topostats.plottingfuncs

"""Plotting data."""
from pathlib import Path
from typing import Union
import logging

from matplotlib.patches import Rectangle, Patch
import matplotlib.pyplot as plt
from mpl_toolkits.axes_grid1 import make_axes_locatable
import numpy as np

from topostats.logs.logs import LOGGER_NAME
from topostats.theme import Colormap

# pylint: disable=too-many-instance-attributes
# pylint: disable=too-many-locals
# pylint: disable=too-many-arguments
# pylint: disable=dangerous-default-value

LOGGER = logging.getLogger(LOGGER_NAME)

# pylint: disable=too-many-instance-attributes
# pylint: disable=too-many-arguments
# pylint: disable=too-many-locals
# pylint: disable=dangerous-default-value


[docs] class Images: """Plots image arrays""" def __init__( self, data: np.array, output_dir: Union[str, Path], filename: str, pixel_to_nm_scaling: float = 1.0, masked_array: np.array = None, title: str = None, image_type: str = "non-binary", image_set: str = "core", core_set: bool = False, pixel_interpolation: Union[str, None] = None, cmap: str = "nanoscope", mask_cmap: str = "jet_r", region_properties: dict = None, zrange: list = [None, None], colorbar: bool = True, axes: bool = True, save: bool = True, save_format: str = "png", histogram_log_axis: bool = True, histogram_bins: int = 200, dpi: Union[str, float] = "figure", ) -> None: """ Initialise the class. Parameters ---------- data : np.array Numpy array to plot. output_dir : Union[str, Path] Output directory to save the file to. filename : Union[str, Path] Filename to save image as. pixel_to_nm_scaling : float The scaling factor showing the real length of 1 pixel, in nm. masked_array : np.ndarray Optional mask array to overlay onto an image. title : str Title for plot. image_type : str The image data type - binary or non-binary. image_set : str The set of images to process - core or all. core_set : bool Flag to identify image as part of the core image set or not. pixel_interpolation: Union[str, None] Interpolation to use (default: None). cmap : str Colour map to use (default 'nanoscope', 'afmhot' also available). mask_cmap : str Colour map to use for the secondary (masked) data (default 'jet_r', 'blu' proivides more contrast). region_properties: dict Dictionary of region properties, adds bounding boxes if specified. zrange : list Lower and upper bound to clip core images to. colorbar: bool Optionally add a colorbar to plots, default is False. axes: bool Optionally add/remove axes from the image. save: bool Whether to save the image. save_format: str Format to save the image as. histogram_log_axis: bool Optionally use a logarithmic y axis for the histogram plots. histogram_bin: int Number of bins for histograms to use. dpi: Union[str, float] The resolution of the saved plot (default 'figure'). """ self.data = data self.output_dir = Path(output_dir) self.filename = filename self.pixel_to_nm_scaling = pixel_to_nm_scaling self.masked_array = masked_array self.title = title self.image_type = image_type self.image_set = image_set self.core_set = core_set self.interpolation = pixel_interpolation self.cmap = Colormap(cmap).get_cmap() self.mask_cmap = Colormap(mask_cmap).get_cmap() self.region_properties = region_properties self.zrange = zrange self.colorbar = colorbar self.axes = axes self.save = save self.save_format = save_format self.histogram_log_axis = histogram_log_axis self.histogram_bins = histogram_bins self.dpi = dpi
[docs] def plot_histogram_and_save(self): """ Plot and save a histogram of the height map Returns ------- fig: plt.figure.Figure Matplotlib.pyplot figure object ax: plt.axes._subplots.AxesSubplot Matplotlib.pyplot axes object """ if self.image_set == "all": fig, ax = plt.subplots(1, 1, figsize=(8, 8)) ax.hist(self.data.flatten().astype(float), bins=self.histogram_bins, log=self.histogram_log_axis) ax.set_xlabel("pixel height") if self.histogram_log_axis: ax.set_ylabel("frequency in image (log)") else: ax.set_ylabel("frequency in image") plt.title(self.title) plt.savefig( (self.output_dir / f"{self.filename}_histogram.{self.save_format}"), bbox_inches="tight", pad_inches=0.5, dpi=self.dpi, ) plt.close() return fig, ax return None
[docs] def plot_and_save(self): """ Plot and save the images with savefig or imsave depending on config file parameters. Returns ------- fig: plt.figure.Figure Matplotlib.pyplot figure object ax: plt.axes._subplots.AxesSubplot Matplotlib.pyplot axes object """ fig, ax = None, None if self.save: if self.image_set == "all" or self.core_set: if self.axes or self.colorbar: fig, ax = self.save_figure() else: if isinstance(self.masked_array, np.ndarray) or self.region_properties: fig, ax = self.save_figure() else: self.save_array_figure() LOGGER.info( f"[{self.filename}] : Image saved to : {str(self.output_dir / self.filename)}" f".{self.save_format}" ) return fig, ax
[docs] def save_figure(self): """ This function saves figures as plt.savefig objects. Returns ------- fig: plt.figure.Figure Matplotlib.pyplot figure object ax: plt.axes._subplots.AxesSubplot Matplotlib.pyplot axes object """ fig, ax = plt.subplots(1, 1, figsize=(8, 8)) shape = self.data.shape if isinstance(self.data, np.ndarray): im = ax.imshow( self.data, extent=(0, shape[1] * self.pixel_to_nm_scaling, 0, shape[0] * self.pixel_to_nm_scaling), interpolation=self.interpolation, cmap=self.cmap, vmin=self.zrange[0], vmax=self.zrange[1], ) if isinstance(self.masked_array, np.ndarray): self.masked_array[self.masked_array != 0] = 1 mask = np.ma.masked_where(self.masked_array == 0, self.masked_array) ax.imshow( mask, cmap=self.mask_cmap, extent=( 0, shape[1] * self.pixel_to_nm_scaling, 0, shape[0] * self.pixel_to_nm_scaling, ), interpolation=self.interpolation, alpha=0.7, ) patch = [Patch(color=self.mask_cmap(1, 0.7), label="Mask")] plt.legend(handles=patch, loc="upper right", bbox_to_anchor=(1, 1.06)) plt.title(self.title) plt.xlabel("Nanometres") plt.ylabel("Nanometres") plt.axis(self.axes) if self.colorbar and self.image_type == "non-binary": divider = make_axes_locatable(ax) cax = divider.append_axes("right", size="5%", pad=0.05) plt.colorbar(im, cax=cax, label="Height (Nanometres)") if self.region_properties: fig, ax = add_bounding_boxes_to_plot(fig, ax, shape, self.region_properties, self.pixel_to_nm_scaling) if not self.axes and not self.colorbar: plt.title("") fig.frameon = False plt.savefig( (self.output_dir / f"{self.filename}.{self.save_format}"), bbox_inches="tight", pad_inches=0, dpi=self.dpi, ) else: plt.savefig((self.output_dir / f"{self.filename}.{self.save_format}"), dpi=self.dpi) else: plt.xlabel("Nanometres") plt.ylabel("Nanometres") self.data.show( ax=ax, extent=(0, shape[1] * self.pixel_to_nm_scaling, 0, shape[0] * self.pixel_to_nm_scaling), interpolation=self.interpolation, cmap=self.cmap, ) plt.close() return fig, ax
[docs] def save_array_figure(self) -> None: """This function saves only the image array as an image using plt.imsave""" plt.imsave( (self.output_dir / f"{self.filename}.{self.save_format}"), self.data, cmap=self.cmap, vmin=self.zrange[0], vmax=self.zrange[1], format=self.save_format, ) plt.close()
[docs] def add_bounding_boxes_to_plot(fig, ax, shape, region_properties: list, pixel_to_nm_scaling: float) -> None: """Add the bounding boxes to a plot. Parameters ---------- fig: plt.figure.Figure Matplotlib.pyplot figure object ax: plt.axes._subplots.AxesSubplot. Matplotlib.pyplot axes object shape: tuple Tuple of the image-to-be-plot's shape. region_properties: Region properties to add bounding boxes from. pixel_to_nm_scaling: float The scaling factor from px to nm. Returns ------- fig: plt.figure.Figure Matplotlib.pyplot figure object. ax: plt.axes._subplots.AxesSubplot Matplotlib.pyplot axes object. """ for region in region_properties: min_y, min_x, max_y, max_x = (x * pixel_to_nm_scaling for x in region.bbox) # Correct y-axis min_y = (shape[0] * pixel_to_nm_scaling) - min_y max_y = (shape[0] * pixel_to_nm_scaling) - max_y rectangle = Rectangle((min_x, min_y), max_x - min_x, max_y - min_y, fill=False, edgecolor="white", linewidth=2) ax.add_patch(rectangle) return fig, ax